Molecular characterization of a maize B chromosome centric sequence.
نویسندگان
چکیده
منابع مشابه
Molecular characterization of a maize B chromosome centric sequence.
Supernumerary chromosomes are widespread in the plant kingdom but little is known of their molecular nature or mechanism of origin. We report here the initial cloning of sequences from the maize B chromosome. Our analysis suggests that many sequences are highly repetitive and shared with the normal A chromosomes. However, all clones selected for B-specificity contain at least one copy of a part...
متن کاملCharacterization of a maize chromosome 4 centromeric sequence: evidence for an evolutionary relationship with the B chromosome centromere.
Previous work has identified sequences specific to the B chromosome that are a major component of the B centromere. To address the issue of the origin of the B and the evolution of centromere-localized sequences, DNA prepared from plants without B chromosomes was probed to seek evidence for related sequences. Clones were isolated from maize line B73 without B chromosomes by screening DNA at red...
متن کاملMolecular and functional dissection of the maize B chromosome centromere.
The centromere of the maize (Zea mays) B chromosome contains several megabases of a B-specific repeat (ZmBs), a 156-bp satellite repeat (CentC), and centromere-specific retrotransposons (CRM elements). Here, we demonstrate that only a small fraction of the ZmBs repeats interacts with CENH3, the histone H3 variant specific to centromeres. CentC, which marks the CENH3-associated chromatin in maiz...
متن کاملCharacterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations.
Maize B chromosome sequences have been previously cloned by microdissection, and all are proven to be highly repetitive, to be homologous to the normal complement, and to show no similarity to any published gene other than mobile elements. In this study, we isolated sequences from defined B regions. The strategy involved identification and then mapping of AFLP-derived B fragments before cloning...
متن کاملMolecular organization of large fragments in the maize B chromosome: indication of a novel repeat.
The supernumerary B chromosome has no apparent effects on plant growth, and its molecular makeup is difficult to unravel, due to its high homology to the normal complement, which prevents conventional cloning. This difficulty was overcome previously by microdissecting the B chromosome under the microscope to result in 19 B clones, one of which is B specific and highly repetitive, dispersing ove...
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ژورنال
عنوان ژورنال: Genetics
سال: 1993
ISSN: 1943-2631
DOI: 10.1093/genetics/135.2.589